ABSTRACT
Objective: To identify the differential proteins from follicular fluid of patients with polycystic ovarian syndrome (PCOS) employing protein chip array, and provide a theoretical basis for further study of PCOS. Methods: AAH-BLG-507 array was used to test the follicular fluid of 6 PCOS patients with normal body mass index (BMI) and 6 non-PCOS controls with age and BMI-matched. The differential proteins were identified and bioinformatics analysis was carried out by functional annotation analysis, KEGG pathway analysis and protein-protein interaction network analysis. Results: Compared with non-PCOS controls, 25 up-regulated proteins and 49 down-regulated proteins were identified from follicular fluid of patients with PCOS. The classification of differential proteins based on molecular function revealed that they were mainly involved in cytokine activity and chemokine activity. KEGG pathway analysis was performed and pathways associated with cytokine-cytokine receptor interaction and chemokine signaling pathway were significantly enriched. The core proteins filtered by protein-protein interaction network analysis were mainly chemokines and their receptors. Conclusion: Protein chip array can be used to establish the differential expressed protein profile from follicular fluid of patients with PCOS. The cytokinecytokine receptor interaction and chemokine signaling pathway may be involved in the pathogenesis of PCOS.
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Objective: To compare the gastric emptying rate in course-matched patients with type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM), and to explore the impact of gastric emptying rate on premeal insulin therapy design in patients with T1DM. Methods: The gastric half-emptying time (T50) and clinical data of 16 patients with T1DM and 32 course-matched patients with T2DM treated at the Department of Endocrinology and Metabolism, Renji Hospital, Shanghai Jiao Tong University School of Medicine from September 2013 to June 2014 were analyzed, retrospectively. The T50 and clinical data of the course-matched patients with T1DM and T2DM were compared. The impact of T50 on premeal insulin therapy design in patients with T1DM was analyzed. Results: The T50 of patients with T1DM was significantly longer than that of the course-matched patients with T2DM (P=0.013). T1DM patients with delayed gastric emptying rate tended to shorten the time between insulin injection and meal than those with normal gastric emptying rate. Conclusion: The gastric emptying rates of patients with T1DM are slower than those of the course-matched patients with T2DM. Measurement of gastric emptying rate not only detects abnormal gastric emptying rate in patients with diabetes but also guides the choice of premeal insulin therapy, optimizes glycemic control and avoids large glucose fluc-tuations in patients with TIDM.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of acupuncture on the morphine-related side effects (nausea, vomiting, itchiness and gastrointestinal disorders) in patients undergoing anesthesia and analgesia and try to find the clinical mechanism of acupuncture.</p><p><b>METHODS</b>Patients scheduled to have transurethral prostatic resection: enrolled in the study (69 patients), characterized as Grade II or III by the American Society of Anesthesiologists physical status classification, were randomly assigned to three groups, 23 patients in each group: the placebo group (control group), non-anesthesia area (NAA) group, and the anesthesia area (AA) group. After spinal anesthesia, all patients received Patients Controlled Epidural Analgesia. The vital signs, bowel sounds, visual analogue scales, itchiness, nausea and vomiting, and time for first exhaust post operation were observed.</p><p><b>RESULTS</b>Altogether, nine cases were eliminated, three in each group. Finally, sixty cases completed this study,: twenty in each group. There was a significant decrease of bowel sounds after morphine spinal anesthesia in the three groups. Compared with the control or AA group, the bowel sound recovered after acupuncture, the incidences of nausea and vomiting, itchiness, and time for first exhaust after operation decreased in the NAA group (P<0.05). There was a significant decrease of the incidence for itchiness in the NAA group compared with <the control group (P<0.05).</p><p><b>CONCLUSIONS</b>On the basis of this study, it is obviously seen that acupuncture could: decrease the incidence of morphine-related side effects (nausea and vomiting, itchiness and gastrointestinal disorders) when the spinal cord conduct is normal. However, it decreases only the incidence of itchiness but not for gastrointestinal dysfunction when the spinal cord is blocked.</p>
Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , Acupuncture Therapy , Analgesia , Analgesia, Epidural , Anesthesia, Epidural , Methods , Anesthesia, Spinal , Methods , Intestines , Morphine , Postoperative Complications , Postoperative Nausea and Vomiting , Therapeutics , Prostatic Hyperplasia , General Surgery , Pruritus , Therapeutics , Transurethral Resection of Prostate , Rehabilitation , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of acupuncture for preventing and treating the complication after Morphine anesthesia and analgesia and to explore the mechanism of acupuncture treatment.</p><p><b>METHODS</b>Patients scheduled to undergo transurethral resection of prostate (TURP) were randomly divided into 3 groups, 20 cases in each group. After lumbar anesthesia, group II and III were treated with acupuncture, and group I with no acupuncture, retaining needle for 30 minutes. Hegu (LI 4), Quchi (LI 11), Neiguan (PC 6), Zhigou (TE 6) were selected in group II, and Zusanli (ST 36), Shangjuxu (ST 37), Xiajuxu (ST 39) and Sanyinjiao (SP 6) selected in group III. The indices of bowel sounds, nausea and vomiting, itching were observed before, during and one day after operation.</p><p><b>RESULTS</b>After lumbar anesthesia with Morphine, the bowel sounds were significant decrease in all the 3 groups. After acupuncture and before TURP the number of times of bowel sound were increased in group II with significant differences as compared with those in group I and group III at the same time point (all P < 0.05). The incidence rates of vomiting and itching were 5.0% (1/20) and 30.0 (6/20) respectively in group II, which were less than 35.0% (7/20) and 80.0% (16/20) in group I.</p><p><b>CONCLUSION</b>Acupuncture has a therapeutic effect on the complication induced by Morphine without nerve block. However, the therapeutic effect of acupuncture is attenuated after nerve block. Its mechanism may be related to nervous pathway.</p>